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Lipid peroxidation products Lipid peroxidation is the key step in the execution of ferroptosis and its metabolites are direct indicators of ferroptotic activity

NuPage LDS sample buffer, supplemented with NuPAGE sample reducing agent (Invitrogen) were added prior to boiling for 10 min at 90 C

Dimeric compounds are promising for simultaneously targeting dual binding domains, enhancing molecular recognition precision and consequently optimizing reaction dynamics and biochemical selectivity 13,14

Analysis was performed in negative and positive ionization mode